The Amino Acid Sequence of the Insoluble Tryptic Peptide,βT10,11,12 from theβ-Polypeptide Chain in Macaca mulatta Monkey Hemoglobin

Globin prepared from macaca mulatta monkey hemoglobin was separated into • @ƒ¿ • @ and ƒÀ-polypeptide • @ chains • @ by • @ countercurrent • @ distribution • @ method. • @ The ƒÀ-polypeptide • @ chain • @was • @digested • @ with • @trypsin. • @ The • @insoluble • @ tryptic • @ peptide (so-called • @ ƒÀ-core), • @ the • @precipitate • @ of • @ the • @digest • @at • @ pH • @6.4, • @was • @obtained • @ by centrifugation. This insoluble tryptic peptide after oxidized with performic acid was digested with pepsin or redigested with trypsin, and the amino acid • @ composition • @ was • @examined. • @ Next, • @ the • @amino • @ acid • @sequence • @ of • @the ƒÀ-core containing 38 amino acid residues was determined by the DNP method, the PTC method, and the carboxypeptidase method. The result was compared with • @ the • @ known • @ sequence • @ of • @the ƒÀ-core • @of • @human • @ hemoglobin. • @ Between these two hemoglobins, two amino acid substitutions were found; that is, the 5th and 22nd residues from the N-terminus are threonine and arginine in case of human hemoglobin, but glutamine and lysine, respectively in case of macaca mulatta monkey hemoglobin.